Triple negative breast carcinoma with normal tissue microarray, containing 102 cases breast carcinoma with ER, PR and Her-2 all negative, 2 each of breast carcinoma with ER+, PR+ and Her-2+, (among them 89 cases invasive carcinoma of no special type, 4 mixed cancer, 8 medullary carcinoma, 2 each of carcinoid and micropapillary carcinoma, 1 each of neuroendocrine carcinoma, aggressive adenocarcinoma and mucinous adenocarcinoma), plus 4 breast tissue, single core per case
Routine histology procedures including Immunohistochemistry (IHC) and In Situ Hybridization (ISH), protocols which can be found at our support page.
1. TMA slides were sectioned and stored at 4°C and may not be fresh cut, but still suitable for IHC. Please request fresh cut if experiment involves phospho-specific antibodies, RNA studies, FISH or ISH, etc. A minimum of 3 slides per TMA must be purchased to cover the cost of trimming for fresh sectioning.
2. Most TMA slides were not coated with an extra layer of paraffin (tissue cores can be easily seen on the glass). To prevent tissue detachment during antigen retrieval, unbaked slides must be baked for at least 30 to 120 minutes at 60°C. before putting into xylene for de-paraffinization. Baked slides were sent out baked for 2 hours.In the following specsheet,“*” means invalid core; “-” means no applicable or negative in IHC markers.
Mouseover and click individual cores to view high resolution images.